Porphyrins and Chlorins from a Congenital Porphyria Patient.

Analysis of porphyrins from the urine, blood, and feces of a congenital porphyria patient showed coproand uroporphyrins from the blood contained more than 95% of the Type I isomer, and those from stool and urine, about 90%. The intermediate hepta-, hexaand pentacarboxylic porphyrins from the urine contained 70% or less of the Type I isomer. The results are discussed and compared with those in porphyria cutanea tarda and from congenitally porphyric cattle. A new tricarboxylic porphyrin, its methyl ester with a melting point of 165#{176} and an absorption band at 627 mJL in chloroform, was isolated from the feces. A urinary chlorin (643 mj ) was separated and studied, and a possible structure of a heptacarboxylic chlorin is suggested by its formation from porphobilinogen and by other pertinent data. Three fecal chiorins (with absorption bands at 646, 660, and 668 mj ) were also isolated. Only the one with 646 m t absorption is relatively stable.